Enzyme Activity Lab Report Text

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Ap biology lab: catalase enzymes in this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and ph along with substrate concentration and enzyme concentration were the conditions tested in the experiment. Four ap biology classes performed this experiment and the data presented in this report will reflect the averages of said classes. In the later discussion sections, it will become very obvious that human error was the deciding factor in the data collection.

Enzymes are biological catalysts that carry out thousands of chemical reactions that occur in living cells. Generally large proteins, enzymes are made up of several hundred amino acids, and often contain a non proteinaceuos group essential in the actual catalyst. There are several factors that contribute to the denaturing of the enzyme that also determine the enzyme's shape. These factors are very closely regulated in both living organisms and in laboratory environments, so as to achieve optimum enzyme activity. Temperature of the enzymatic reaction or enzyme itself will help in dictating the activity rate of the function.

The other factors include the substrate concentration a substrate is the substance being acted upon and enzyme concentration. The ap biology classes performed this study, to examine the effects of changes in the optimum conditions for enzyme activity. Procedure a disc of filter paper was immersed into the enzyme solution for about 15 seconds. Then it was removed from the solution and blotted on a paper towel for 5 seconds. Next the disc was placed in a beaker filled with the hydrogen peroxide substrate and allowed to sink to the bottom of the beaker. The time measured was that from the time the disc reached the bottom of the beaker until it touched the surface of the hydrogen peroxide.

This procedure was carried out for all four of the conditions being tested and the rates were recorded in respective data tables. An average rate of all four classes, that performed the experiment, was then graphed. Data and analysis 100 0.081 0.654 0.057 0.13 0.201 80 0.087 0.109 0.043 0.173 0.103 75 0.181 0.057 0.05 0.127 0.104 60 0.095 0.038 0.047 0.1 0.073 50 0.131 0.034 0.041 0.098 0.076 25 0.077 0.014 0.028 0.067 0.047 10 0.064 0.011 0.026 0.042 0.036 0 no rx no rx no rx no rx no rx as the enzyme concentration increased so too did the activity rate. Substrate concentration 0% no rx no rx no rx no rx no rx 0.20% 0.025 0.019 0.01 0.011 0.016 0.30% 0.04 0.021 0.014 0.015 0.023 0.50% 0.058 0.018 0.02 0.024 0.03 0.80% 0.041 0.038 0.04 0.035 0.039 1.00% 0.1 0.031 0.042 0.051 0.059 as the substrate concentration increased so too did the activity rate in an almost linear fashion. We measured the amount of enzyme in centimeters cm and bubble height in millimeters mm. The amount of enzyme are in ranges as follows: tube 1 1cm 5cm, tube 2: 2cm 6cm, and tube 3: 3cm 7cm. Tube 2 had a ph of 7 and the activity in this particular on was a bubble height of 30 mm.

Overall the data tells us that neither acidic or basic ph levels cause a change, but the exception would be a moderate level would cause some change. Introduction: what would happen to your cells if they made a poisonous chemical? you might think that they would die. They do not die because your cells use enzymes to break down these poisonous chemicals into harmless substances. Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. Each of these enzymes is responsible for one particular reaction that occurs in the cell. In this lab, you will study an enzyme that is found in the cells of many living tissues.

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The name of the enzyme is catalase kat uh layss it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances water and oxygen. Light can also break down h2 o2 which is why the chemical is sold in dark containers. This reaction is important to cells because hydrogen peroxide is produced as a byproduct of many normal cellular reactions.

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If the cells did not break down the hydrogen peroxide, they would be poisoned and die. This is possible because when a cell dies, the enzymes remain intact and active for several weeks, as long as the tissue is kept refrigerated. Biology labs 108537 views 1170 words enzymes are proteins that are critical to catalyzing reactions brooker, widmaier, graham amp stiling, 2011.

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